Search results for "methods [Biological Assay]"
showing 10 items of 243 documents
NOseq: amplicon sequencing evaluation method for RNA m6A sites after chemical deamination
2020
Abstract Methods for the detection of m6A by RNA-Seq technologies are increasingly sought after. We here present NOseq, a method to detect m6A residues in defined amplicons by virtue of their resistance to chemical deamination, effected by nitrous acid. Partial deamination in NOseq affects all exocyclic amino groups present in nucleobases and thus also changes sequence information. The method uses a mapping algorithm specifically adapted to the sequence degeneration caused by deamination events. Thus, m6A sites with partial modification levels of ∼50% were detected in defined amplicons, and this threshold can be lowered to ∼10% by combination with m6A immunoprecipitation. NOseq faithfully d…
iGEMS: an integrated model for identification of alternative exon usage events.
2016
Abstract DNA microarrays and RNAseq are complementary methods for studying RNA molecules. Current computational methods to determine alternative exon usage (AEU) using such data require impractical visual inspection and still yield high false-positive rates. Integrated Gene and Exon Model of Splicing (iGEMS) adapts a gene-level residuals model with a gene size adjusted false discovery rate and exon-level analysis to circumvent these limitations. iGEMS was applied to two new DNA microarray datasets, including the high coverage Human Transcriptome Arrays 2.0 and performance was validated using RT-qPCR. First, AEU was studied in adipocytes treated with (n = 9) or without (n = 8) the anti-diabe…
Human voice pitch measures are robust across a variety of speech recordings: methodological and theoretical implications
2021
Fundamental frequency ( f o ), perceived as voice pitch, is the most sexually dimorphic, perceptually salient and intensively studied voice parameter in human nonverbal communication. Thousands of studies have linked human f o to biological and social speaker traits and life outcomes, from reproductive to economic. Critically, researchers have used myriad speech stimuli to measure f o and infer its functional relevance, from individual vowels to longer bouts of spontaneous speech. Here, we acoustically analysed f o in nearly 1000 affectively neutral speech utterances (vowels, words, counting, greetings, read paragraphs and free spontaneous speech) produced by the same 154 men and women, ag…
Contour integration with corners.
2016
Contour integration refers to the ability of the visual system to bind disjoint local elements into coherent global shapes. In cluttered images containing randomly oriented elements a contour becomes salient when its elements are coaligned with a smooth global trajectory, as described by the Gestalt law of good continuation. Abrupt changes of curvature strongly diminish contour salience. Here we show that by inserting local corner elements at points of angular discontinuity, a jagged contour becomes as salient as a straight one. We report results from detection experiments for contours with and without corner elements which indicate their psychophysical equivalence. This presents a challeng…
Démarche statistique pour la sélection des indicateurs par Random Forests pour la surveillance de la qualité des sols
2013
The volume of data, and the large number of biological variables to be tested (one hundred), require analytical techniques, such asRandom Forests, which can overcome the problem of multi-colinearity for the selection of indicators, sensitive to various factors.Random Forests methodology is appropriate for the selection of the most discriminant variables. So, we searched for the best wayto select them, by bringing together all biological variables, representing the Microflora and Fauna. This approach focuses on impactindicators from the Bio2 program, indicators of flora and indicators of accumulation (snails) were not included.This work has been implemented on the three factors of discrimina…
Simulazione numerica tridimensionale del campi de moto indotti dal vento
2006
Nell’articolo viene proposto un modello numerico tridimensionale al volume finiti, che viene utilizzato per la simulazione di campi di moto indotti dal mento in corpi idrici con batimetria complessa. I risultati numerici sono confrontati con misure sperimentali. Al fine di verificare gli effetti sul calcolo del campi idrodinamici di alcume ipotesi simplificative, relative alla distribuzione verticale della pressione e degli sforzi di Reynolds, sono stati quindi condotti alcuni test numerici, dai quali si è evidenziato che l’ipotesi di distribuzione idrostatica delle pressioni, cui si fa ricorso nelle approssinazioni di tipo “quasi-3D”, può essere utilizzata per il carcolo del profili vertic…
The role of fire within Neolithic collective burials: Spatial analyses of cremains from the site of La Truie Pendue, France
2016
International audience; The use of collective graves is one of the main features of the western European Late Neolithic. A single gravesite received the successive deposition of dozens or sometimes hundreds of individuals. While cremations or even full-fired inhumation layers are often found within these funerary deposits, the actual role of fire is still poorly understood. Recently discovered within the important archaeological complex of Passy (Yonne, France), the burned collective grave of La Truie-Pendue provides an outstanding case study to examine the use of fire within Neolithic funerary rites. In this study, we develop a new contextual approach to bone alterations in order to recons…
The effectiveness of compositional animation design: Evidence from eye tracking
2017
Communication donnée le 1er septembre 2017 lors du symposium : Eye tracking as a method in learning and testing with different representations(session L 8); International audience; Learners have difficulty in decomposing conventionally designed animations to obtain raw material suitable for building high quality mental models. A composition approach to designing animations based on the Animation Processing Model was developed as a principled alternative to prevailing approaches. It provides learners with pre-decomposed material that is structured and sequenced to facilitate the relation building required for effective mental model construction. Study of a compositional animation that presen…
Predicting dengue fever outbreaks in French Guiana using climate indicators
2016
Background Dengue fever epidemic dynamics are driven by complex interactions between hosts, vectors and viruses. Associations between climate and dengue have been studied around the world, but the results have shown that the impact of the climate can vary widely from one study site to another. In French Guiana, climate-based models are not available to assist in developing an early warning system. This study aims to evaluate the potential of using oceanic and atmospheric conditions to help predict dengue fever outbreaks in French Guiana. Methodology/Principal Findings Lagged correlations and composite analyses were performed to identify the climatic conditions that characterized a typical e…
Coherence between brain activation and speech envelope at word and sentence levels showed age-related differences in low frequency bands
2021
Abstract Speech perception is dynamic and shows changes across development. In parallel, functional differences in brain development over time have been well documented and these differences may interact with changes in speech perception during infancy and childhood. Further, there is evidence that the two hemispheres contribute unequally to speech segmentation at the sentence and phonemic levels. To disentangle those contributions, we studied the cortical tracking of various sized units of speech that are crucial for spoken language processing in children (4.7–9.3 years old, N = 34) and adults (N = 19). We measured participants’ magnetoencephalogram (MEG) responses to syllables, words, and…